Author Rhea Mehta, PhD
The use of mushrooms in traditional ancient therapies dates back to at least the Neolithic Age (in China, 10,000 B.C. – 2,000 B.C). For millennia, mushrooms have been regarded and revered by humans as edible and medicinal agents. Ancient Asian traditions have stressed the importance of several mushroom species, most notably the lingzhi or reishi, lion’s mane, cordyceps, and shitake mushroom.
The history of mushroom consumption is rich in Tibetan shamanism and Buddhism, as well as in spiritual cultures of Mesoamerica, Mexico, and Guatemala with the use of hallucinogenic mushroom species. Use of medicinal mushrooms has a long history in Russia, Europe, and some parts of Africa, including Nigeria, Algeria, and Egypt.
Given the use of mushrooms in traditional therapies spanning the globe, modern researchers are attempting to validate and document some of the ancient knowledge. In the past three decades, the integration of knowledge and methods from different disciplines of science that study mushrooms, known as mycology, has demonstrated the nutritional (culinary) and therapeutic (medicinal) value of many compounds extracted from a range of mushrooms species .
"Today, more than 100 therapeutic applications have been demonstrated by mushrooms..."
Traditionally, mushrooms were used to maintain good health, as well as to prevent and treat diseases, mainly by regulating the immune system. In the last decade scientists have identified and explored other ways in which various mushroom species promote good health, revealing mushrooms to beunique, multi-modal medicinal agents worthy of further investigation.
Today, more than 100 therapeutic applications have been demonstrated by mushrooms, including antioxidant, anti-inflammatory, anticancer, antitumor, anti-diabetic, antiviral, antibacterial, antiparasitic, and antifungal, ability to modulate the immune system, and lower plasma cholesterol levels. Mushrooms also protect against brain, heart, and liver damage. While modern researchers are just beginning to explore the clinical potential of medicinal mushrooms in the West, countries in the East such as Japan, Korea, China, and Russia have already adopted preparations derived from mushrooms for use in clinical practice.
For example, mushrooms have been used in traditional Chinese medicine for years to treat pulmonary diseases, and in Japan, the mushroom T. versicolor has been used as an approved product for adjunctive cancer treatment since the 1970s.
The number of mushroom species on Earth is currently estimated at 150,000, yet perhaps only 10 percent are known to science. This presents researchers with an exciting opportunity to dive into this vast and largely untapped field of mushroom science with hopes of uncovering new and potentially breaththrough nutritional and therapeutic agents.
What are mushrooms?
Mushrooms comprise an extremely abundant and diverse world of fungi. They are something special in the living world, being neither plant nor animal. Belonging to a kingdom of their own, called Myceteae, all mushrooms are heterotrophic, meaning they cannot make their own food. Instead, they absorb nutrients from other sources of organic carbon such as complex organic polymers like cellulose, which get degraded by extracellular enzymes secreted by mushrooms.
Taxonomically, mushrooms can be classified into two different categories: Basidiomycetes, which comprise many of the well-known genera, and Ascomycetes. The structures that we refer to as mushrooms are in fact only the fruiting bodies of fungi.
The vegetative parts of fungi, called mycelia, are a system of cordlike strands that branch out of soil, compost, wooden logs, and other biomass materials that are host to fungi. After some time, the matured mycelia growing on such materials produce the fruiting bodies we know as mushrooms.
As mentioned, mushrooms have been used by humans for thousands of years as food and medicine. More than 14,000 mushroom species are recognized by science, and among them, about 2,000 are classified as edible. These edible mushrooms can be categorized into 30 genera, with 270 species that bear potential therapeutic value in human health.
Medicinal mushrooms possess physiological benefits and medicinal characteristics in addition to taste and aroma. They are a rich source of nutrients such as carbohydrates, fibers, proteins, unsaturated fats, vitamins, and minerals. They also produce secondary metabolites, which are largely responsible for their therapeutic effects. These metabolites are bioactive, low-molecular-weight compounds that are produced in response to stress as a defense and signaling mechanism for survival. Interestingly, they are not required by the fungi for normal growth and reproduction.
More than 100 species of medicinal mushrooms are used in Asia. Some of the more commonly used species are introduced below. It is worth noting that while these mushrooms have been used historically as traditional medicines, with many being used clinically today in Asia, modern research is insufficient to draw conclusions, as it has mostly been conducted in cellular and animal studies.
While many of these medicinal mushrooms have been recognized by scientists for their health-promoting activities, pharmaceutical application in healthcare is still far away in the majority of countries across the world, and especially in the West.
Today, medicinal mushrooms can be found as dietary and nutritional supplements. They are often recommended by nutritionists and holistic healthcare professionals and sold by health food and wellness retailers. Given the lack of consensus and limited information on safety, it is a good idea to consult with your healthcare professional about appropriate dosages and risk factors with certain medications. Finally, when choosing medicinal mushrooms as dietary supplements, it is important to ensure they have been tested by a third-party facility for quality and purity and that there is sufficient information available about the product.
Reishi (Ganoderma lucidum)
Ganoderma lucidum (G. lucidum), also known as reishi in Japanese Medicine or lingzhi in Chinese Medicine, is a large, dark mushroom from Asian Traditional Medicine that has been praised for its powerful health benefits for over 2,000 years. It was in the 16th-century Ming Dynasty when G. lucidum was discovered to have therapeutic value. Benefits of reishi included enhanced vital energy, improved memory, and improved cardiac function, and tonifying and anti-aging effects.
According to the State Pharmacopoeia of the People’s Republic of China, G. lucidum acts to replenish Qi, calm the mind, and reduce coughing and asthma. It is also recommended for insomnia, dizziness, shortness of breath, and heart palpitations. Since the early 1970s, cultivation of G. lucidum has become a major source of the mushroom, with popularity extending outside of Asian Traditional Medicine and into the West.
Lion's mane (Hericium erinaceus)
Hericium erinaceus (H. erinaceus) is an edible mushroom with distinguished medicinal value, a long history of use in Traditional Chinese Medicine, and an established process of cultivation in Asia since the late 1980s. H. erinaceus is referenced by several names, including yamabushitake in Japanese Medicine, houtou in Chinese Medicine, or more broadly as lion’s mane mushroom.
Lion’s mane is considered a saprotroph, or weak parasite, and grows on dead wood and occasionally on the cracks of living hardwood across Asia, Europe, and North America. More recently, it has earned scientific attention as a potential source for a variety of therapies for cancer, depression, diabetes, and diseases of the heart and nervous system.
Shiitake (Lentinula edodes)
eThe shiitake mushroom (Lentinula edodes) is the second most commonly consumed mushroom worldwide and has grown to become an extensively used culinary ingredient. Historically, therapeutic use of the L. edodes became widespread in the Ming Dynasty after methods of cultivating the fungus on logs were developed.
Shiitake was traditionally used as a tonic to improve circulation and vigor. Health-promoting properties included improved heart health and efficacy against lung disease and intestinal worms. It was also associated with the treatment of chronic rheumatism and cancer. Today lentinan, one of the active ingredients purified from shiitake mushrooms, has been used experimentally and clinically to enhance immune responses in the treatment of advanced gastric cancer in Japan.
Turkey tail (Trametes versicolor)
Trametes versicolor (T. versicolor) or Coriolus versicolor (C. versicolor), also known as yun zhi in Traditional Chinese Medicine and kawaratake in Japanese Medicine, is a woody bracket polypore mushroom that grows on dead logs throughout the world in many diverse climates, including North America. The name turkey tail refers to its concentric rings of brown and tan, which resemble turkey tail feathers.
Turkey tail mushroom has a long history of treasured use in Asia as food and in Asian Traditional Medicine as a therapeutic agent. In 1965, the immunomodulatory activity of turkey tail was identified in Japan by a chemical engineer who observed a case of cancer remission after ingesting it. Subsequent research and findings led to its clinical use as a complementary therapeutic agent in cancer treatments in Japan and China.
Chaga (Inonotus obliquus)
Chaga mushroom (Inonotus obliquus) grows as a rock-hard protrusion from birch trees in cooler climates, such as Northern Europe, Siberia, Russia, Korea, Northern Canada, and Alaska. Chaga is also referenced by other names, such as cinder conk, birch canker polymore, clinker polypore, and black mass. Unlike the other commonly known medicinal mushrooms, this dense structure is known as a sclerotium rather than a fruiting body.
For centuries, chaga powder was used as traditional medicine in tea form in Russia and other Northern European countries to boost the immune system and improve overall health. It has also been used in the treatment of some cancers, diabetes, and heart disease.
Cordyceps is a genus of parasitic fungi that grows on the larvae of insects. These mushrooms remain dormant in the soil until they attack their host, replacing its tissue and sprouting long stems that grow outside the infected host’s body.
With over 750 identified species, cordyceps have been used for centuries in Traditional Chinese Medicine as a tonic, food, and medicine. However, it was in the early 1990s that cordyceps gained worldwide attention, when Chinese longdistance runners who took tonic from the species C. sinensis broke world records during their training periods.
In recent times, several cordyceps species have been shown to have medicinal value, with the most studied being C. militaris and C. sinensis. They have the potential to help treat respiratory, liver, kidney, and heart and lung diseases, as well as type 2 diabetes and some cancers.
Maitake (Grifola frondosa)
Maitake, known as Grifola frondosa (G. frondosa), is an edible medicinal mushroom with a large fruiting body characterized by overlapping caps that grows at the base of stumps tree trunks of dead, dying, or aging hardwoods such as oaks, elms, and chestnuts. It is also known as hen of the woods in North America or maitake in Japan. Maitake is mainly found in northern temperate regions of Japan, as well as in some European countries and the Northeastern United States.
G. frondosa is treasured in both Traditional Japanese and Chinese Medicine as a medicinal mushroom to boost energy and improve spleen and stomach ailments, as well as to calm the mind. Cultivation began in Japan in the early 1980s, with China and the United States beginning large-scale production a decade later.
The nutritional value of mushrooms typically comes from high protein, low fat, high fiber, and diverse vitamin and mineral content. Notably, mushrooms are abundant in essential amino acids leucine, valine, glutamine, and glutamic and aspartic acids. Fatty acids found in mushrooms are mainly linoleic, oleic, and palmitic fatty acids. Carbohydrates include chitin, glycogen, trehalose, and mannitol, as well as fiber enriched sources, β-glucans, hemicelluloses, and pectic substances.
Minerals found in high quantities in mushrooms include potassium, phosphorus, magnesium, calcium, copper, iron, selenium, and zinc. Abundant vitamins include riboflavin (vitamin B2), niacin, folates, and traces of vitamins C, B1, B12, D, and E. Mushrooms are the only non-animal food source of vitamin D and therefore the only food-based vitamin D source for vegetarians.
Major bioactive compounds
Medicinal mushrooms are a rich source of bioactive compounds, which are found in mushroom fruiting bodies, spores, cultured mycelia, and cultured broth. These compounds, also referred to as secondary metabolites, are largely responsible for the therapeutic properties of the mushrooms. They include polysaccharides, glycosides, alkaloids, volatile oils, terpenoids, tocopherols, phenolics, flavonoids, carotenoids, and lactones.
The most important secondary metabolites are polysaccharides, which belong to the 1,3-β-glucans family. Polysaccharide β-glucans or polysaccharideprotein complexes in mushroom extracts have immense therapeutic potential in human health, as they demonstrate many health-promoting benefits, including anti-diabetic, anticancer, anti-obesity, modulating the immune system, lowering plasma cholesterol levels, preventing liver damage, neuroprotective, antibiotic, detoxifying, and anti-aging properties.
"...medicinal mushrooms may be useful to both human health through drug development and environmental health through biotechnological processes."
The ability to modulate the immune system and the antitumor activity of polysaccharides or polysaccharideprotein complexes is worth highlighting because research in this area is quite extensive. Clinical trials of several mushroom polysaccharide compounds as treatment for various cancers and other diseases have been conducted in Asia. To date, over 120 medicinal functions have been identified, with the majority of research still in exploratory phase in cellular models.
New proteins with biological activities have also been found in medicinal mushrooms which may be useful to both human health through drug development and environmental health through biotechnological processes. These include biomass- degrading enzymes, lectins, proteases and protease inhibitors, ribosome-inactivating proteins, and hydrophobins.
Polysaccharides are the most well-researched secondary metabolites of medicinal mushrooms, with data having been collected from hundreds of different species of the higher basidiomycetes genus. As mentioned, they are best known for their antitumor capacity and ability to modulate the immune system.
Specifically, in a tumor-bearing mouse model, mushroom polysaccharides were shown to activate the immune response of the host organism, resulting in a 50% reduction in tumor size and an increase in survival. The underlying mechanism of action of polysaccharides therefore points to its ability to modulate the immune system, versus a direct killing of tumor cells.
Given β-glucans are not synthesized by humans, they are not recognized by the human immune system. Therefore, upon consumption, they induce both innate and adaptive immune responses as a protective strategy. In doing so, they protect the body from pathogenic microbes, viruses and harmful environmental toxins and carcinogens that have entered the body. This health-promoting activity can be beneficial for patients with compromised immune systems, such as those recovering from chemotherapy.
β-glucans are the main polysaccharides found in mushrooms, with about half of the mushroom cell wall mass made up of β-glucans. This has important implications for the industrial use of these compounds, given that many are excreted into the cell growth medium, resulting in efficiencies around recovery, purification, and characterization.
Recent research has brought attention to the presence of another bioactive compound abundant in mushrooms: terpenoids. Terpenoids are now among the most potent of secondary metabolites found in medicinal mushrooms, best known for their anticancer, antitumor, antimicrobial, and neuroprotective benefits. Terpenoids demonstrate a spectrum of therapeutic properties with great medicinal potential.
Phenolic compounds are another group of secondary metabolites worth highlighting, as they exhibit a wide range of therapeutic functions including anti-inflammatory, anti- allergenic, anti-atherogenic, antimicrobial, antithrombotic, cardioprotection and vasodilating benefits. The chief mechanism of action of this group of compounds is related to their antioxidant activity.
Studies have shown that medicinal mushrooms have over 125 therapeutic functions. Some of the major benefits are described below.
Anticancer and Immune Function
Cancer is one of the leading causes of death worldwide, with the most common treatment being chemotherapy, which has serious side effects. The search continues for effective, non-toxic treatments.
In recent years, several species of medicinal mushrooms have been studied for their anticancer properties in human cell lines, including leukemia, breast, renal, gastric, colorectal, and cervical cancers. Mushroom polysaccharides have been identified as one of the major categories of bioactive compounds to demonstrate anticancer effects in several cancer cell lines, including colorectal, renal, and oral cancer. Other bioactive compounds have also been recognized for their therapeutic potential, such as lectins (leukaemia), triterpenes (breast cancer), and clitocine (cervical cancer).
In the medicinal mushroom Pleurotus eryngii (eringi), the anticancer effects demonstrated by polysaccharides include increased activity of natural killer cells and cytotoxic T lymphocytes in the spleen, as well as an increase in tumor necrosis factor (TNF-α) and interleukin-2 in a renal cancer mouse model, thereby pointing to immune system modulation as the underlying mechanism of action.
In another study, G. lucidum (reishi) was reported to increase cellular autophagy and the formation of autophagosomes in a gastric cancer cell line, demonstrating a different mechanism of action involving the removal of damaged cells.
"...we are only at the very beginning of uncovering the clinical potential of medicinal mushrooms..."
While the beneficial effects of medicinal mushrooms on boosting cellular immunity and protecting against various types of cancer have been well documented, we are only at the very beginning of uncovering the clinical potential of medicinal mushrooms, with much to consider, including extraction methods of bioactive compounds, mushroom cultivation, and safety.
Antiviral and antibacterial
Human immunodeficiency virus type 1 (HIV-1) is a highly contagious virus affecting millions of people worldwide. Current treatment for HIV-1 postpones the development of acquired immunodeficiency syndrome (AIDS), which is the disease caused by HIV-1. Research in this field continues, and medicinal mushrooms are showing exciting albeit early developments in this space.
Proteins, triterpenoids, peptides, lectins, and polysaccharide-protein complexes have all been shown to possess antiviral properties against HIV-1. One reported mechanism of action by G. lucidum (reishi) includes the inhibition of the HIV-1 reverse transcriptase enzyme, which plays a critical role in the lifecycle of the virus.
The antimicrobial activity of several medicinal mushrooms has also recently been investigated. In G. lucidum, polysaccharides were shown to inhibit the growth of pathogenic bacteria and microbes found in food, including Bacillus cereus, Bacillus subtilis, Escherichia coli, Aspergillus niger and Rhizopus nigricans.
Anti-diabetic and anti-obesity
Diabetes mellitus, also known as type 2 diabetes, is a chronic metabolic disease impacting millions of people worldwide. It is defined by high blood glucose levels caused by imbalances with the insulin hormone. Polysaccharides extracted from a number of different medicinal mushroom species have been associated with reducing blood glucose levels, and for some, in increasing the secretion of insulin by β-cells, which lowers blood glucose levels.
In one study, bioactive substances in an extract of the G. frondosa (maitake) fruiting body decreased blood glucose levels in genetically-born diabetic mice. Some medicinal mushrooms with alleged antidiabetic properties are commercially available as dietary supplements, including products containing cordyceps, lion’s mane, and maitake. Maitake, along with other medicinal mushrooms, have also shown promising anti-obesity effects, likely due to the activity of β-glucans in the mushroom extracts.
"Medicinal mushrooms possess antioxidant and anti-inflammatory properties, which have been associated with many of the health-promoting properties of fungi."
Antioxidant, anti-inflammatory, and anti-aging
Medicinal mushrooms possess antioxidant and anti-inflammatory properties, which have been associated with many of the healthpromoting properties of fungi. G. lucidum (reishi) has been reported to increase the levels of antioxidative enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in liver and brain homogenates of mice sera.
Medicinal mushrooms have also demonstrated anti-inflammatory activity by suppressing the production of different types of inflammatory mediators, including nitric oxide, nitric oxide synthase, nuclear factor kappa beta (NF-κΒ), tumor necrosis factor (TNF)-α, and other pro-inflammatory cytokines.
Anti-aging effects have been demonstrated in G. lucidum through the activity of ergosterols, secondary metabolites obtained from the mushroom. These compounds have been shown to extend the lifespan of the pathogen Saccharomyces cerevisiae by regulating the expression of an oxidative stress-responsive gene. Polysaccharides in G. lucidum and other medicinal mushrooms have also been shown to prevent oxidative stress and inhibit certain enzymes responsible for aging, thereby delaying it.
Heart disease is marked by high levels of low-density lipoprotein (LDL) or bad cholesterol, and low levels of high-density lipoprotein (HDL), or good cholesterol. Trans fats are primarily responsible for increasing cholesterol and triglyceride levels in the body, and other risk factors include high blood pressure and high glucose levels.
Several medicinal mushrooms have been associated with reducing total cholesterol levels in the body, including G. frondosa (maitake), H. erinaceus (lion’s mane), Pleurotus eryngii (eringi), and Hypsizygus marmoreus (bunashimeji). Various mechanisms of action have been identified, such as the inhibition of platelet aggregation, the inhibition of angiotensin, the decrease in very low-density (VLDL) lipoproteins, and improvements in lipid metabolism, with peptides being the major bioactive compounds responsible for these beneficial properties.
Cultivation and production
Mushroom cultivation has many steps, including selection of mushroom fruiting culture, preparation of spawn and substrate/compost, inoculation of substrate, crop care, and finally, harvesting, handling, and proper storage of the mushrooms. There are three different categories of decomposers within the basidiomycetes genus, which represent a continuum in the metabolic transition from biomass and other organic waste materials to soil in the context of large-scale mushroom production.
Primary decomposers (e.g., oyster and shiitake mushrooms) degrade cellulose and lignin from dead wood/logs, leaves, straw, cotton seed hulls, corn cobs or peanut shells, and do not depend on other organisms or their metabolites. Secondary decomposers (e.g., button mushrooms) colonize composted materials, and tertiary decomposers (e.g., agrocybe) are found in the soil.
Today, indoor cultivation using artificial logs is a more common method for large-scale production. One method involves the use of plastic bags filled with sawdust-based substrates infused with nutrients. When the bag is colonized with mycelia, holes are poked through to enable fruiting to occur.
As our world continues to increase in population and our collective behavior continues to harm our planet, we face three main issues: the shortage of real food, an increase in health issues and chronic diseases, and environmental deterioration.
Fascinatingly, mushrooms can address all three concerns.
It is important to remember that mushrooms, like all other fungi, lack chlorophyll. As such, they can be referred to as non-green organisms since they cannot convert solar energy to organic matter like plants do. They can, however, convert massive amounts of biomass waste produced from agriculture and forestry activities into dietary food, medicine, animal feed, and crop fertilizer. Further, they can help clean and restore soil that is damaged from chemical agricultural methods.
Environmental sustainability and regeneration
Mushroom cultivation is a powerful tool to restore, replenish, and remediate our planet’s over-burdened ecosphere. Because cultivation can take place on organic waste, this presents an exciting opportunity to biologically recycle the high amounts of accumulated natural by-products of the agro-food industry, which contribute to environmental pollution through their burning and also take up precious land space that could otherwise be used to grow crops.
Natural byproducts of the agro-food industry include dead wood and leaves, which hold several nutrients that cannot be accessed unless broken down and digested. Fungi are the only organisms in the world that can decompose such organic waste products. The process is actually done by mycelia, the vegetative part of fungi, which resembles white threads that grow from the dead wood and leaves.
These mycelia release powerful extracellular enzymes and acids that can break down cellulose and lignin, the two key components of plant fiber. In the natural ecosystem, once the process of decomposition starts, organisms from other kingdoms as well as nutrients present in the organic waste can now participate in and speed up the degradation process.
The organic waste—now bio-recycled to soil through the process of mushroom cultivation—can be transformed into nutritious fertilizer or feed, useful for growing crops, feeding livestock, and for remediating soil and water damaged through chemical agricultural practices. At the same time, the mushrooms harvested through this process can be used for both nutritional (protein-enriched) and medicinal purposes, as previously described.
Current state of research
Medicinal mushroom science has made good progress over the last three decades, resulting in new classes of pharmaceutical agents in Asia and various types of dietary and nutritional supplements across the world. The community of mushroom researchers and thought leaders has grown extensively during this time as well, owing to the rich opportunities in this field of research, both from a human health and environmental perspective.
More than 600 studies on medicinal mushrooms have been published worldwide, and numerous human clinical trials have been conducted. Many bioactive compounds have completed the clinical trial phases in Asia and are being used to treat various cancers and other diseases.
Dietary supplements and pharmaceutical drugs
Medicinal mushrooms have much potential as daily dietary or nutritional supplements to benefit overall health and immune function. Today, several types of mushroom supplements are available.
As pharmaceutical agents, medicinal mushrooms are most helpful for modulating immune function and maintaining good health for those with weakened immune systems and those undergoing cancer treatment. Major bioactive compounds that have been developed as products include polysaccharides, specifically β-glucans.
The following products are available for clinical and commercial purposes:
• krestin (PSK) and polysaccharide peptide (PSP) from Trametes versicolor
• lentinan isolated from Lentinula edodes
• schizopyllan (sonifilan, sizofiran, or SPG) from Schizophyllum commune
• befungin from Inonotus obliquus
• D-fraction from Grifola frondosa
• GLPS polysaccharide fraction from Ganoderma lucidum
Given the many therapeutic activities of the secondary metabolites of various medicinal mushrooms, they remain a relatively unclaimed source for drug discovery.
Recently, the interest in developing mushroom extracts to be marketed as dietary supplements and novel prebiotics (non-digestive β-glucans) has grown. As popularity increases, questions arise about safety, standardization, regulation, efficacy, and mechanism of action.
Given that no reputable standards or protocols are currently in place for the production, extraction, and testing of medicinal mushroom bioactive products, quality and efficacy between products vary greatly.
Other critical safety questions also remain unanswered, such as are medicinal mushroom products safe for pregnant women, or can they be consumed by young children, given immune systems in young children are not yet mature? Concerns around dosage also exist, given the discrepancy between various forms and formulations.
Many clinical trials have determined that 500–1000mg broken down and taken over the course of the day (2-3 times) is the acceptable dosage of medicinal mushroom preparations. According to Traditional Chinese Medicine, the standard daily dose of medicinal mushrooms per day in different forms (tablets, capsules, liquid extracts, etc.) must be equivalent to approximately 100–150g of fresh mushroom material.
From a research perspective, the majority of studies with medicinal mushrooms have been performed on cellular and animal models. More clinical data from human studies is therefore required to clarify whether the health-promoting claims reported are valid and significant. Critical factors going forward include ensuring studies are of the highest quality, following manufacturing best practices, and developing sustainable, large-scale production of medicinal mushrooms under controlled conditions.
Medicinal mushrooms and their bioactive compounds have the potential to act as promising source materials for both the dietary supplement and pharmaceutical industries. Medicinal mushroom cultivation can also serve to promote equitable economic growth in communities within the developing world that are subject to the accumulation of large amounts of agricultural crop residues and forest industry wastes.
What’s more, mushroom cultivation has the power to restore and regenerate soil health in a zero emissions manner, thereby positively contributing to not only human health and social change, but also to healing our planet.
The multidimensional nature of the powerful fungi is highly unique and worth celebrating, even in its current state of research and level of impact. This is just the beginning for the abundant and diverse mushroom kingdom. I, for one, am deeply excited about the vast opportunities within this space.
1. Chang ST, Miles PG, Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact, second edition, CRC Press, Boca Raton, 2004;385- 387.
2. Cheung PC, Mushrooms as Functional Foods John Wiley & Sons, 2008, Technology & Engineering.
3. Money, NP, Are mushrooms medicinal?, Fungal Biology, 2016;120(4):449-453.
4. Valverde ME, Hernández-Pérez T, Paredes-López O, Edible Mushrooms: Improving Human Health and Promoting Quality Life, Int J Microbiol. 2015:376- 387.
5. Guillamón E, García-Lafuente A, Lozano M, et al., Edible mushrooms: role in the prevention of cardiovascular diseases. Fitoterapia. 2010;81(7):715-723.
6. Hobbs C, Medicinal Mushrooms: An Exploration of Tradition, Healing, and Culture, Edible mushrooms, 2002.
7. Jones K, Shiitake: A Major Medicinal Mushroom, Alternative and Complementary Therapies, 2009:4(1).
8. Nguyen AH, Maria MS, Gonzaga BS, Lim VM, Clinical features of shiitake dermatitis: a systematic review, International Journal of Dermatology, 2017.
9. PDQ Integrative, Medicinal Mushrooms (PDQ®) Health Professional Version, Alternative, and Complementary Therapies Editorial Board, 2020.
10. Deepalakshmi, Mirunalini, Therapeutic properties and current medical usage of mushroom: Ganoderma Lucidum, IJPSR, 2011;2(8):1922-1929.
11. Standish LJ, Wenner CA, Sweet ES, et al., Trametes versicolor mushroom immune therapy in breast cancer. J Soc Integr Oncol. 2008;6(3):122-128.
12. Kubo K, Aoki H, Nanba H, Anti-diabetic Activity Present in the Fruit Body of Grifola Frondosa (Maitake). Biol Pharm Bull, 1994:17(8):1106-1110.
13. Zhong XH, Ren K, Lu SJ, Yang SY, Sun DZ, Progress of research on Inonotus obliquus. Chin J Integr Med. 2009;15(2):156-160.
14. Opeyemi JO, Tang J, Tola A, et al., The genus Cordyceps: An extensive review of its traditional uses, phytochemistry and pharmacology, 2018;129:293-316.
15. Cör D, Knez Ž, Knez Hrnčič M. Antitumour, Antimicrobial, Antioxidant and Antiacetylcholinesterase Effect of Ganoderma Lucidum Terpenoids and Polysaccharides: A Review. Molecules. 2018;23(3):649.
16. He Y, Li X, Hao C, et al., Grifola frondosa polysaccharide: a review of antitumor and other biological activity studies in China. Discov Med. 2018;25(138):159-176.
17. He X, Wang X, Fang J, et al., Polysaccharides in Grifola frondosa mushroom and their health promoting properties: A review. Int J Biol Macromol. 2017;101:910- 921. Medicinal Mushrooms in the 21st Century 25
18. Erjavec J, Kos J, Ravnikar M, et al., Proteins of Higher Fungi—From Forest to Application, Trends Biotechnol, 2012;5:259-73.
19. Dasgupta A, Acharya K, Mushrooms: an emerging resource for therapeutic terpenoids, 3 Biotech 2019;9:369.
20. Chaturvedi VK, Agarwal S, Gupta KK, et al., Medicinal mushroom: boon for therapeutic applications, 3 Biotech, 2018;8:334.
21. McIntosh M, Stone BA, Stanisich VA, Curdlan and other bacterial (13)-β-D glucans, Applied Microbiology and Biotechnology, 2005;68(2):163-173.
22. Vetvicka V, Vannucci L, Sima P, Richter J. Beta Glucan: Supplement or Drug? From Laboratory to Clinical Trials. Molecules. 2019;24(7):1251.
23. Muszyńskaa B, Grzywacz-Kisielewskaa A, Katarzyna K, Gdula-Argasińskab J, Anti-inflammatory properties of edible mushrooms: A review, Food Chemistry, 2018;243(15):373-381. 24. Cör D,, Knez Z, Hrnčič KM Antitumour, Antimicrobial, Antioxidant and Antiacetylcholinesterase Effect of Ganoderma Lucidum Terpenoids and Polysaccharides: A Review, Molecules 2018;23:649.
25. Wasser SP, Medicinal Mushrooms as a Source of Antitumor and Immunomodulating Polysaccharides, Appl Microbiol Biotechnol, 2002;60(3):258- 74.
26. Chen W, Tan H, Liu Q, et al., A Review: The Bioactivities and Pharmacological Applications of Phellinus linteus, Molecules 2019;24:1888.
27. Balasundramab N, Kalyana N, Sundramb N, Sammana S, Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses, Food Chemistry Analytical, Nutritional and Clinical Methods, 2006;99(1):191-203.
28. Chung-Hua H, Kung-Chang H, Yi-Hsiung C, and Pesus C, The Mushroom Agaricus blazei Murill Extract Normalizes Liver Function in Patients with Chronic Hepatitis B, The Journal of Alternative and Complementary Medicine, 2008;14(3):299-301.
29. Stamets P. Growing gourmet and medicinal mushrooms. New York: Crown Publishing Group, 1993.
30. Jan S. Tkacz, Lene Lange, Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine, Springer Science & Business Media, 2004.
31. Rahman MA , Abdullah N, Aminudin N, Interpretation of mushroom as a common therapeutic agent for Alzheimer’s disease and cardiovascular diseases, Crit Rev Biotechnol, 2016;36(6):1131-1142.
32. Sullivan, R, et al., Medicinal Mushrooms and Cancer Therapy: translating a traditional practice into Western medicine, Perspectives in Biology and Medicine, 2006;49(2):159- 170. Project MUSE.
33. Wasser SP, Int J Med Mushrooms, Medicinal Mushrooms in Human Clinical Studies. Part I. Anticancer, Oncoimmunological, and Immunomodulatory Activities: A Review, 2017;19(4):279-317. Medicinal Mushrooms in the 21st Century 26
34. Phan CW, David P, Naidu M, Wong KH, Sabaratnam V. Therapeutic potential of culinary-medicinal mushrooms for the management of neurodegenerative diseases: diversity, metabolite, and mechanism. Crit Rev Biotechnol, 2015;35(3):355-368.
35. Zhang JJ, Li Y, Zhou T, et al., Bioactivities and Health Benefits of Mushrooms Mainly from China, Molecules, 2016;21(7):938.
36. Zhang H, Wang ZY, Zhang Z, Wang X, Purified Auricularia auricular-judae polysaccharide (AAP I-a) prevents oxidative stress in an ageing mouse model, Carbohydrate Polymers, 2011;84(1):638-648.
37. Weng Y, Xiang L, Matsuura A, et al., Ganodermasides A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma lucidum on yeast via UTH1 gene, Bioorg Med Chem, 2010;18(3):999-1002.
38. Reis F, Lima RT, Morales P, et al., Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells, Molecules, 2015;20(10):17872-17882.
39. Zeng Y, Yang A, Xu JB, et al., Antitumor activity of a polysaccharide from Pleurotus eryngii on mice bearing renal cancer, Carbohydrate Polymers, 2013;95(2):615-620.
40. Chang ST, The World Mushroom Industry: Trends and Technological Development, Int J Med Mushr, 2006;8(4):10:297-314.
41. Grimm D, Wösten HAB, Mushroom cultivation in the circular economy, Appl Microbiol Biotechnol. 2018;102(18):7795-7803.
42. Rhodes CJ, Applications of bioremediation and phytoremediation, Science Progress, 2013;96(4):417–427.
43. Rhodes CJ, Mycoremediation (bioremediation with fungi) – growing mushrooms to clean the earth, Chemical Speciation and Bioavailability 2014;26(3):2047- 6523. 44. Wasser SP, Current findings, future trends, and unsolved problems in studies of medicinal mushrooms, Appl Microbiol Biotechnol, 2011;89:1323-1332.
45. Wasser SP, Medicinal Mushroom Science: Current Perspectives, Advances, Evidences, and Challenges, Biomed J, 2014;37:345-356.
46. Chang ST, Waser SP, Current and Future Research Trends in Agricultural and Biomedical Applications of Medicinal Mushrooms and Mushroom Products (Review), International Journal of Medicinal Mushrooms, 2018;20(12):1121-1133.
47. Muhammad BL, Suleiman B, Global Development of Mushroom Biotechnology, International Journal of Emerging Trends in Science and Technology, 2015;2(6):2660- 2669.
48. Dhar BL, Shrivastava N, Mushrooms and Environmental Sustainability, Mushroom Research Development and Training Centre (MRDTC), 2012.
49. Ahmed I, Syed AR, Economic viability of mushrooms cultivation to poverty reduction in bangladesh, Tropical and Subtropical Agroecosystems, 2008;8(1):93-99.